【报告题目】Biocompatible and Photostable AIE Nanoprobes for Multiphoton Functional Bioimaging

【报告人】钱骏博士

【邀请人】贺号博士

【时间】1月16日（周一）10:00-11:00

【地点】Med-X 108

【摘要】Relying on simultaneous absorption of two or more near-infrared photons by a fluorophore, multiphoton microscopy can achieve deep tissue penetration and efficient light detection noninvasively, which makes it very suitable for thick-tissue and in vivo bioimaging. However, most conventional organic fluorophores suffer from severe aggregation-caused quench (ACQ) effect, which means their fluorescence is easily quenched at aggregate or solid state. In 2001, Tang and coworkers observed a diametrically opposite phenomenon called “aggregation-induced emission” (AIE), which provides an ideal solution to the notorious ACQ problem. Based on this mechanism, hydrophobic AIE molecules can be encapsulated with various types of nanoparticles, or form nanoaggregates in an aqueous solution spontaneously. AIE nanoparticles have bright fluorescence, large multiphoton absorption cross-section, and are very resistant to photobleaching even under high-power laser irradiation, which facilitates their applications in long-term multiphoton microscopy as nanoprobes. In this talk, I will introduce some exemplary applications of AIE nanoparticles in two- and three-photon in vivo functional brain imaging of mice, such as brain-microglia imaging, 3D architecture reconstruction of brain blood vessel, and blood velocity measurement. We also applied AIE nanoparticles in three-photon imaging of zebrafish at different growth stages.

【报告人简介】钱骏，博士，男，浙江大学光电科学与工程学院教授，博士生导师。2004年6月在浙江大学光电系获学士学位，2009年6月在浙江大学光电系获博士学位，2009年7月至2011年6月在浙江大学光及电磁波研究中心从事博士后研究，2011年6月至2011年12月在浙江大学光电系担任讲师，2012年1月至2015年12月在光电系担任副教授，2012年1月成为浙江大学求是青年学者、担任博士生导师，2016年1月至今在光电学院担任教授。2006年至2007年曾在美国纽约州立大学布法罗分校“激光、光子学、生物光子学研究中心”进行学术访问，师从纳米生物光子学的国际领军人物Paras N. Prasad教授。主要从事纳米生物光子学的研究。已发表SCI 论文50多篇,其中，以第一/通讯作者身份在Adv. Mater., Angew Chem Int Edit, ACS Nano, Biomaterials, Nano Research, Theranostics, Optics. Lett.,Biomed. Opt. Express., J. Biomed. Opt.等高水平期刊上发表论文30多篇。著有英文学术专著一章节。承担了国家自然科学基金（面上和青年），973、863子课题，浙江省杰出青年科学基金等项目。